Recombinant Human Cystathionine γ-Lyase/CTH Protein

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032319-10, PKSH032319-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cystathionine γ-Lyase; CTH

Target Synonym: Cystathionine Gamma-Lyase; Cysteine-Protein Sulfhydrase; Gamma-Cystathionase; CTH

UNIProt ID: P32929

Research Areas: Signal Transduction; Cancer; metabolism;

Target Species: Human

Expression Host: E.coli

Fusion Tag: None

Accession: P32929

Background: Cystathionine Gamma-Lyase (CTH) belongs to the trans-sulfuration enzymes family. CTH exists as a homotetramer and interacts with CALM in a calcium-dependent manner. CTH breaks down cystathionine into cysteine, ammonia and 2-oxobutanoate. CTH catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine and has broad substrate specificity. Defects in CTH will lead to cystathioninuria, which is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine.

Sequence: Met 1-Ser405

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 20% Glycerol, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.7 kDa

Observed MW: 50 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only